Platelets that are rich in plasma are the natural healing mechanism that the body uses to trigger the regeneration of the damaged or dead cells in the body. This is used as a treatment for curing many issues in people such as skin problems, dental issues, bone and cartilage problems, and so on.
Collecting PRP Sample
Platelet-rich plasma is the plasma that is rich in the platelet cells in the required numbers. It is extracted from the blood of the patients, and hence the first step is taking a blood sample from the patient’s body. This procedure includes using some tubes that are filled with anticoagulant chemicals to make sure that the sample is not allowed to clot after extracting from the patient’s body.
The tubes that are used in PRP therapy are known as PRP tubes, and you can buy PRP tubes by visiting the official webpage of Dr. Renat Akhmerov’s Plasmolifting Technologies. They are known for their best-quality PRP tubes and can guarantee to get you the best product for PRP therapy of all kinds. Visit their website to get more information.
Optimal Centrifugation Protocols
With the help of the differential centrifugation system, it is possible to separate the platelets and plasma from all the other components in the layers inside the PPR tubes. However, the purity of PRP cannot be obtained if the viability and integrity of platelet and plasma are preserved during the separation process. Hence, using the right centrifugation protocol becomes mandatory here.
The centrifugation protocol is as follows.
Selecting the centrifuge model
This is an important step that should be followed during the PRP preparation process. Better separation of PRP can be achieved in the models that spin horizontally. This allows the components in the blood sample to separate from one another based on the density of every component. This is the ideal choice for reducing the chances of cell trauma during the centrifugation procedure.
Temperature maintenance
The optimum temperature that should be followed during the centrifugation procedure is not more than 24 degrees Celsius and less than 20 degrees Celsius. However, the experts suggest keeping the centrifugation temperature between 12 and 16 degrees so that there is an improved and viable recovery of platelets from the blood sample.
Single or Double Spin Centrifugation
Some centrifugation systems are designed with the double spin mechanism to help the researchers get the purest form of any liquid that they need to conduct their experiments. Such centrifugation systems are ideal for PRP procedure too, as the resulting PRP will be of the highly concentrated solution of platelets.
Required Force During Centrifugation
The solution of 400g when spun for about 10 minutes in the centrifugation system can get the 5 times pure results as required by the users. The same goes for centrifugation of the blood samples too. The same force can be implied during PRP centrifugation to get 5 times platelet concentration.
If the samples are kept for more than a few hours during the PRP therapy, then it is suggested to keep them in a storage unit at room temperature. However, the samples should not be stored for more than 8 hours.
